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Expression, purification, crystallization and preliminary crystallographic analysis of the phosphoglycerate kinase from Acinetobacter baumannii.

ABSTRACT: Acinetobacter baumannii is a common multidrug-resistant clinical pathogen that is often found in hospitals. The A. baumannii phosphoglycerate kinase (AbPGK) is involved in the key energy-producing pathway of glycolysis and presents a potential target for antibiotic development. AbPGK has been expressed and purified; it was crystallized using lithium sulfate as the precipitant. The AbPGK crystals belonged to space group P222(1). They diffracted to a resolution of 2.5?Å using synchrotron radiation at the Canadian Light Source.

SUBMITTER: Baretta K

PROVIDER: S-EPMC3388923 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature

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Expression, purification, crystallization and preliminary crystallographic analysis of the phosphoglycerate kinase from Acinetobacter baumannii.

Baretta Kayla K Garen Craig C Yin Jiang J James Michael N G MN

Acta crystallographica. Section F, Structural biology and crystallization communications 20120627 Pt 7

Acinetobacter baumannii is a common multidrug-resistant clinical pathogen that is often found in hospitals. The A. baumannii phosphoglycerate kinase (AbPGK) is involved in the key energy-producing pathway of glycolysis and presents a potential target for antibiotic development. AbPGK has been expressed and purified; it was crystallized using lithium sulfate as the precipitant. The AbPGK crystals belonged to space group P222(1). They diffracted to a resolution of 2.5 Å using synchrotron radiation ...[more]

PMID: 22750866

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