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PRR5L degradation promotes mTORC2-mediated PKC-? phosphorylation and cell migration downstream of G?12.


ABSTRACT: Mammalian target of rapamycin complex 2 (mTORC2) phosphorylates AGC protein kinases including protein kinase C (PKC) and regulates cellular functions such as cell migration. However, its regulation remains poorly understood. Here we show that lysophosphatidic acid (LPA) induces two phases of PKC-? hydrophobic motif phosphorylation. The late phase is mediated by G?(12), which specifically activates ARAF, leading to upregulation of the RFFL E3 ubiquitin ligase and subsequent ubiquitylation and degradation of the PRR5L subunit of mTORC2. Destabilization of PRR5L, a suppressor of mTORC2-mediated hydrophobic motif phosphorylation of PKC-?, but not AKT, results in PKC-? hydrophobic motif phosphorylation and activation. This G?(12)-mediated signalling pathway for mTORC2 regulation is critically important for fibroblast migration and pulmonary fibrosis development.

SUBMITTER: Gan X 

PROVIDER: S-EPMC3389271 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature

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PRR5L degradation promotes mTORC2-mediated PKC-δ phosphorylation and cell migration downstream of Gα12.

Gan Xiaoqing X   Wang Jiyong J   Wang Chen C   Sommer Eeva E   Kozasa Tohru T   Srinivasula Srinivasa S   Alessi Dario D   Offermanns Stefan S   Simon Melvin I MI   Wu Dianqing D  

Nature cell biology 20120520 7


Mammalian target of rapamycin complex 2 (mTORC2) phosphorylates AGC protein kinases including protein kinase C (PKC) and regulates cellular functions such as cell migration. However, its regulation remains poorly understood. Here we show that lysophosphatidic acid (LPA) induces two phases of PKC-δ hydrophobic motif phosphorylation. The late phase is mediated by Gα(12), which specifically activates ARAF, leading to upregulation of the RFFL E3 ubiquitin ligase and subsequent ubiquitylation and deg  ...[more]

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