Unknown

Dataset Information

0

The occludin and ZO-1 complex, defined by small angle X-ray scattering and NMR, has implications for modulating tight junction permeability.


ABSTRACT: Tight junctions (TJs) are dynamic cellular structures that are critical for compartmentalizing environments within tissues and regulating transport of small molecules, ions, and fluids. Phosphorylation-dependent binding of the transmembrane protein occludin to the structural organizing protein ZO-1 contributes to the regulation of barrier properties; however, the details of their interaction are controversial. Using small angle X-ray scattering (SAXS), NMR chemical shift perturbation, cross-saturation, in vitro binding, and site-directed mutagenesis experiments. we define the interface between the ZO-1 PDZ3-SH3-U5-GuK (PSG) and occludin coiled-coil (CC) domains. The interface is comprised of basic residues in PSG and an acidic region in CC. Complex formation is blocked by a peptide (REESEEYM) that corresponds to CC residues 468-475 and includes a previously uncharacterized phosphosite, with the phosphorylated version having a larger effect. Furthermore, mutation of E470 and E472 reduces cell border localization of occludin. Together, these results localize the interaction to an acidic region in CC and a predominantly basic helix V within the ZO-1 GuK domain. This model has important implications for the phosphorylation-dependent regulation of the occludin:ZO-1 complex.

SUBMITTER: Tash BR 

PROVIDER: S-EPMC3390843 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature

altmetric image

Publications

The occludin and ZO-1 complex, defined by small angle X-ray scattering and NMR, has implications for modulating tight junction permeability.

Tash Brian R BR   Bewley Maria C MC   Russo Mariano M   Keil Jason M JM   Griffin Kathleen A KA   Sundstrom Jeffrey M JM   Antonetti David A DA   Tian Fang F   Flanagan John M JM  

Proceedings of the National Academy of Sciences of the United States of America 20120618 27


Tight junctions (TJs) are dynamic cellular structures that are critical for compartmentalizing environments within tissues and regulating transport of small molecules, ions, and fluids. Phosphorylation-dependent binding of the transmembrane protein occludin to the structural organizing protein ZO-1 contributes to the regulation of barrier properties; however, the details of their interaction are controversial. Using small angle X-ray scattering (SAXS), NMR chemical shift perturbation, cross-satu  ...[more]

Similar Datasets

| S-EPMC3695765 | biostudies-literature
| S-EPMC2132714 | biostudies-literature
2023-05-10 | PXD041011 | Pride
| S-EPMC2742868 | biostudies-other
| S-EPMC2629239 | biostudies-literature
| S-EPMC2915885 | biostudies-literature
| S-EPMC2168087 | biostudies-literature
| S-EPMC3768107 | biostudies-literature
| S-EPMC3198696 | biostudies-literature
| S-EPMC3832373 | biostudies-literature