Substrate spectrum extension of PenA in Burkholderia thailandensis with a single amino acid deletion, Glu168del.
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ABSTRACT:
SUBMITTER: Yi H
PROVIDER: S-EPMC3393396 | biostudies-literature | 2012 Jul
REPOSITORIES: biostudies-literature
Publications
Substrate spectrum extension of PenA in Burkholderia thailandensis with a single amino acid deletion, Glu168del.
Yi Hyojeong H Kim Karan K Cho Kwang-Hwi KH Jung Oksung O Kim Heenam Stanley HS
Antimicrobial agents and chemotherapy 20120507 7
We describe a deletion mutation in a class A β-lactamase, PenA, of Burkholderia thailandensis that extended the substrate spectrum of the enzyme to include ceftazidime. Glu168del was located in a functional domain called the omega loop causing expansion of the space in the loop, which in turn increased flexibility at the active site. This deletion mutation represents a rare but significant alternative mechanical path to substrate spectrum extension in PenA besides more common substitution mutati ...[more]
Similar Datasets
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