Phosphoinositide-3-kinase, catalytic, alpha polypeptide RNA interference inhibits growth of colon cancer cell SW948.
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ABSTRACT:
SUBMITTER: Huang WS
PROVIDER: S-EPMC3396200 | biostudies-literature | 2012 Jul
REPOSITORIES: biostudies-literature
Publications
Phosphoinositide-3-kinase, catalytic, alpha polypeptide RNA interference inhibits growth of colon cancer cell SW948.
Huang Wen-Sheng WS Wang Tian-Bao TB He Yao Y Chen Yu-Jun YJ Zhong Shi-Long SL Tan Min M
World journal of gastroenterology 20120701 26
<h4>Aim</h4>To investigate the gene knock-down effect by the phosphoinositide-3-kinase, catalytic, alpha polypeptide (PIK3CA)-targeted double-stranded RNA (dsRNA) and its effect on cell proliferation and cycle distribution in SW948.<h4>Methods</h4>Two PIK3CA-targeted dsRNAs were constructed and transfected into SW948 cells. Transfections were performed using lipofectamine™ 2000. The transfection effectiveness was calculated basing on the rate of fluorescence cell of SW948 at 6 h after transfecti ...[more]
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