Ontology highlight
ABSTRACT:
SUBMITTER: Kuhn PH
PROVIDER: S-EPMC3400020 | biostudies-literature | 2012 Jun
REPOSITORIES: biostudies-literature
Kuhn Peer-Hendrik PH Koroniak Katarzyna K Hogl Sebastian S Colombo Alessio A Zeitschel Ulrike U Willem Michael M Volbracht Christiane C Schepers Ute U Imhof Axel A Hoffmeister Albrecht A Haass Christian C Roßner Steffen S Bräse Stefan S Lichtenthaler Stefan F SF
The EMBO journal 20120622 14
Cell surface proteolysis is essential for communication between cells and results in the shedding of membrane-protein ectodomains. However, physiological substrates of the contributing proteases are largely unknown. We developed the secretome protein enrichment with click sugars (SPECS) method, which allows proteome-wide identification of shedding substrates and secreted proteins from primary cells, even in the presence of serum proteins. SPECS combines metabolic glycan labelling and click chemi ...[more]