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Correlation between in vivo biofilm formation and virulence gene expression in Escherichia coli O104:H4.


ABSTRACT: The emergence of novel pathogens poses a major public health threat causing widespread epidemics in susceptible populations. The Escherichia coli O104:H4 strain implicated in a 2011 outbreak in northern Germany caused the highest frequency of hemolytic uremic syndrome (HUS) and death ever recorded in a single E. coli outbreak. Therefore, it has been suggested that this strain is more virulent than other pathogenic E. coli (e.g., E. coli O157:H7). The E. coli O104:H4 outbreak strain possesses multiple virulence factors from both Shiga toxin (Stx)-producing E. coli (STEC) and enteroaggregative E. coli (EAEC), though the mechanism of pathogenesis is not known. Here, we demonstrate that E. coli O104:H4 produces a stable biofilm in vitro and that in vivo virulence gene expression is highest when E. coli O104:H4 overexpresses genes required for aggregation and exopolysaccharide production, a characteristic of bacterial cells residing within an established biofilm. Interrupting exopolysaccharide production and biofilm formation may therefore represent effective strategies for combating future E. coli O104:H4 infections.

SUBMITTER: Al Safadi R 

PROVIDER: S-EPMC3405000 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

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Correlation between in vivo biofilm formation and virulence gene expression in Escherichia coli O104:H4.

Al Safadi Rim R   Abu-Ali Galeb S GS   Sloup Rudolph E RE   Rudrik James T JT   Waters Christopher M CM   Eaton Kathryn A KA   Manning Shannon D SD  

PloS one 20120725 7


The emergence of novel pathogens poses a major public health threat causing widespread epidemics in susceptible populations. The Escherichia coli O104:H4 strain implicated in a 2011 outbreak in northern Germany caused the highest frequency of hemolytic uremic syndrome (HUS) and death ever recorded in a single E. coli outbreak. Therefore, it has been suggested that this strain is more virulent than other pathogenic E. coli (e.g., E. coli O157:H7). The E. coli O104:H4 outbreak strain possesses mul  ...[more]

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