Dataset Information

Schisandrin B attenuates cancer invasion and metastasis via inhibiting epithelial-mesenchymal transition.

ABSTRACT:

Background

Metastasis is the major cause of cancer related death and targeting the process of metastasis has been proposed as a strategy to combat cancer. Therefore, to develop candidate drugs that target the process of metastasis is very important. In the preliminary studies, we found that schisandrin B (Sch B), a naturally-occurring dibenzocyclooctadiene lignan with very low toxicity, could suppress cancer metastasis.

Methodology

BALB/c mice were inoculated subcutaneously or injected via tail vein with murine breast cancer 4T1 cells. Mice were divided into Sch B-treated and control groups. The primary tumor growth, local invasion, lung and bone metastasis, and survival time were monitored. Tumor biopsies were examined immuno- and histo-pathologically. The inhibitory activity of Sch B on TGF-? induced epithelial-mesenchymal transition (EMT) of 4T1 and primary human breast cancer cells was assayed.

Principal findings

Sch B significantly suppressed the spontaneous lung and bone metastasis of 4T1 cells inoculated s.c. without significant effect on primary tumor growth and significantly extended the survival time of these mice. Sch B did not inhibit lung metastasis of 4T1 cells that were injected via tail vein. Delayed start of treatment with Sch B in mice with pre-existing tumors did not reduce lung metastasis. These results suggested that Sch B acted at the step of local invasion. Histopathological evidences demonstrated that the primary tumors in Sch B group were significantly less locally invasive than control tumors. In vitro assays demonstrated that Sch B could inhibit TGF-? induced EMT of 4T1 cells and of primary human breast cancer cells.

Conclusions

Sch B significantly suppresses the lung and bone metastasis of 4T1 cells via inhibiting EMT, suggesting its potential application in targeting the process of cancer metastasis.

SUBMITTER: Liu Z

PROVIDER: S-EPMC3405072 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

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Publications

Schisandrin B attenuates cancer invasion and metastasis via inhibiting epithelial-mesenchymal transition.

Liu Zhen Z Zhang Biao B Liu Kun K Ding Zonghui Z Hu Xun X

PloS one 20120725 7

<h4>Background</h4>Metastasis is the major cause of cancer related death and targeting the process of metastasis has been proposed as a strategy to combat cancer. Therefore, to develop candidate drugs that target the process of metastasis is very important. In the preliminary studies, we found that schisandrin B (Sch B), a naturally-occurring dibenzocyclooctadiene lignan with very low toxicity, could suppress cancer metastasis.<h4>Methodology</h4>BALB/c mice were inoculated subcutaneously or inj ...[more]

PMID: 22848381

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Dataset Information

Schisandrin B attenuates cancer invasion and metastasis via inhibiting epithelial-mesenchymal transition.

Background

Methodology

Principal findings

Conclusions

Publications

Schisandrin B attenuates cancer invasion and metastasis via inhibiting epithelial-mesenchymal transition.

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