Unknown

Dataset Information

0

The intercellular synchronization of Ca2+ oscillations evaluates Cx36-dependent coupling.


ABSTRACT: Connexin36 (Cx36) plays an important role in insulin secretion by controlling the intercellular synchronization of Ca(2+) transients induced during stimulation. The lack of drugs acting on Cx36 channels is a major limitation in further unraveling the molecular mechanism underlying this effect. To screen for such drugs, we have developed an assay allowing for a semi-automatic, fluorimetric quantification of Ca(2+) transients in large populations of MIN6 cells. Here, we show that (1) compared to control cells, MIN6 cells with reduced Cx36 expression or function showed decreased synchrony of glucose-induced Ca(2+) oscillations; (2) glibenclamide, a sulphonylurea which promotes Cx36 junctions and coupling, increased the number of synchronous MIN6 cells, whereas quinine, an antimalarial drug which inhibits Cx36-dependent coupling, decreased this proportion; (3) several drugs were identified that altered the intercellular Ca(2+) synchronization, cell coupling and distribution of Cx36; (4) some of them also affected insulin content. The data indicate that the intercellular synchronization of Ca(2+) oscillations provides a reliable and non-invasive measurement of Cx36-dependent coupling, which is useful to identify novel drugs affecting the function of ?-cells, neurons, and neuron-related cells that express Cx36.

SUBMITTER: Bavamian S 

PROVIDER: S-EPMC3405138 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

altmetric image

Publications

The intercellular synchronization of Ca2+ oscillations evaluates Cx36-dependent coupling.

Bavamian Sabine S   Pontes Helena H   Cancela José J   Charollais Anne A   Startchik Sergei S   Van de Ville Dimitri D   Meda Paolo P  

PloS one 20120725 7


Connexin36 (Cx36) plays an important role in insulin secretion by controlling the intercellular synchronization of Ca(2+) transients induced during stimulation. The lack of drugs acting on Cx36 channels is a major limitation in further unraveling the molecular mechanism underlying this effect. To screen for such drugs, we have developed an assay allowing for a semi-automatic, fluorimetric quantification of Ca(2+) transients in large populations of MIN6 cells. Here, we show that (1) compared to c  ...[more]

Similar Datasets

| S-EPMC10411970 | biostudies-literature
| S-EPMC3320976 | biostudies-literature
| S-EPMC4334551 | biostudies-literature
| S-EPMC7168262 | biostudies-literature
| S-EPMC3127187 | biostudies-literature
| S-EPMC8017953 | biostudies-literature
| S-EPMC5742114 | biostudies-literature
| S-EPMC4223640 | biostudies-literature
| S-EPMC4494757 | biostudies-literature
| S-EPMC1184555 | biostudies-literature