Ontology highlight
ABSTRACT:
SUBMITTER: Xi J
PROVIDER: S-EPMC3405174 | biostudies-literature | 2012 Aug
REPOSITORIES: biostudies-literature
Xi Jiajie J Liu Yan Y Liu Huisheng H Chen Hong H Emborg Marina E ME Zhang Su-Chun SC
Stem cells (Dayton, Ohio) 20120801 8
By sequentially applying sonic hedgehog (C25II) and CHIR99021 (GSK3β inhibitor) to induce the midbrain floor plate (FP) progenitors and fibroblast growth factor 8 (FGF8) to promote dopaminergic differentiation in a chemically defined medium, we have established a robust system for the generation of midbrain dopamine (DA) neurons from human and rhesus monkey embryonic stem cells and induced pluripotent stem cells (PSCs). We found that CHIR99021 specifies diencephalon to hind brain fates in a conc ...[more]