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FolX from Pseudomonas aeruginosa is octameric in both crystal and solution.

ABSTRACT: FolX encodes an epimerase that forms one step of the tetrahydrofolate biosynthetic pathway, which is of interest as it is an established target for important drugs. Here we report the crystal structure of FolX from the bacterial opportunistic pathogen Pseudomonas aeruginosa, as well as a detailed analysis of the protein in solution, using analytical ultracentrifugation (AUC) and small-angle X-ray scattering (SAXS). In combination, these techniques confirm that the protein is an octamer both in the crystal structure, and in solution.

SUBMITTER: Gabrielsen M

PROVIDER: S-EPMC3405516 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature

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FolX from Pseudomonas aeruginosa is octameric in both crystal and solution.

Gabrielsen Mads M Beckham Katherine S H KS Cogdell Richard J RJ Byron Olwyn O Roe Andrew J AJ

FEBS letters 20120324 8

FolX encodes an epimerase that forms one step of the tetrahydrofolate biosynthetic pathway, which is of interest as it is an established target for important drugs. Here we report the crystal structure of FolX from the bacterial opportunistic pathogen Pseudomonas aeruginosa, as well as a detailed analysis of the protein in solution, using analytical ultracentrifugation (AUC) and small-angle X-ray scattering (SAXS). In combination, these techniques confirm that the protein is an octamer both in t ...[more]

PMID: 22575651

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