Recombinase polymerase amplification assay for rapid detection of Francisella tularensis.
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ABSTRACT:
SUBMITTER: Euler M
PROVIDER: S-EPMC3405570 | biostudies-literature | 2012 Jul
REPOSITORIES: biostudies-literature
Publications
Recombinase polymerase amplification assay for rapid detection of Francisella tularensis.
Euler Milena M Wang Yongjie Y Otto Peter P Tomaso Herbert H Escudero Raquel R Anda Pedro P Hufert Frank T FT Weidmann Manfred M
Journal of clinical microbiology 20120418 7
Several real-time PCR approaches to develop field detection for Francisella tularensis, the infectious agent causing tularemia, have been explored. We report the development of a novel qualitative real-time isothermal recombinase polymerase amplification (RPA) assay for use on a small ESEQuant Tube Scanner device. The analytical sensitivity and specificity were tested using a plasmid standard and DNA extracts from infected rabbit tissues. The assay showed a performance comparable to real-time PC ...[more]
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