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Rapid detection of human mastadenovirus species B by recombinase polymerase amplification assay.


ABSTRACT: BACKGROUND:As an important component of the causative agent of respiratory tract infections, enteric and eye infections, Human mastadenoviruses (HAdVs) species B spread easily in the crowd. In this study, we developed a recombinase polymerase amplification (RPA) assay for rapidly detecting HAdVs species B which was comprised of two different formats (real-time and lateral-flow device). RESULTS:This assay was confirmed to be able to detect 5 different HAdVs species B subtypes (HAdV-B3, HAdV-B7, HAdV-B11, HAdV-B14 and HAdV-B55) without cross-reactions with other subtypes and other respiratory tract pathogens. This RPA assay has not only highly sensitivity with low detection limit of 50 copies per reaction but also short reaction time (

SUBMITTER: Wu T 

PROVIDER: S-EPMC6325725 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature

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Rapid detection of human mastadenovirus species B by recombinase polymerase amplification assay.

Wu Tao T   Wu Haizhen H   Zhao Kangchen K   Hu Chaoyou C   Ge Yiyue Y   Zhu Xiaojuan X   Zhang Xingchen X   Zhou Minghao M   Zhu Fengcai F   Cui Lunbiao L  

BMC microbiology 20190108 1


<h4>Background</h4>As an important component of the causative agent of respiratory tract infections, enteric and eye infections, Human mastadenoviruses (HAdVs) species B spread easily in the crowd. In this study, we developed a recombinase polymerase amplification (RPA) assay for rapidly detecting HAdVs species B which was comprised of two different formats (real-time and lateral-flow device).<h4>Results</h4>This assay was confirmed to be able to detect 5 different HAdVs species B subtypes (HAdV  ...[more]

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