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Rapid spoligotyping of Mycobacterium tuberculosis complex bacteria by use of a microarray system with automatic data processing and assignment.


ABSTRACT: Membrane-based spoligotyping has been converted to DNA microarray format to qualify it for high-throughput testing. We have shown the assay's validity and suitability for direct typing from tissue and detecting new spoligotypes. Advantages of the microarray methodology include rapidity, ease of operation, automatic data processing, and affordability.

SUBMITTER: Ruettger A 

PROVIDER: S-EPMC3405571 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature

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Rapid spoligotyping of Mycobacterium tuberculosis complex bacteria by use of a microarray system with automatic data processing and assignment.

Ruettger Anke A   Nieter Johanna J   Skrypnyk Artem A   Engelmann Ines I   Ziegler Albrecht A   Moser Irmgard I   Monecke Stefan S   Ehricht Ralf R   Sachse Konrad K  

Journal of clinical microbiology 20120502 7


Membrane-based spoligotyping has been converted to DNA microarray format to qualify it for high-throughput testing. We have shown the assay's validity and suitability for direct typing from tissue and detecting new spoligotypes. Advantages of the microarray methodology include rapidity, ease of operation, automatic data processing, and affordability. ...[more]

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