Spoligotyping of Mycobacterium tuberculosis Complex Isolates by Use of Ligation-Based Amplification and Melting Curve Analysis.
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ABSTRACT:
SUBMITTER: Zeng X
PROVIDER: S-EPMC5005503 | biostudies-literature | 2016 Sep
REPOSITORIES: biostudies-literature
Publications
Spoligotyping of Mycobacterium tuberculosis Complex Isolates by Use of Ligation-Based Amplification and Melting Curve Analysis.
Zeng Xiaohong X Li Hui H Zheng Rongrong R Kurepina Natalia N Kreiswirth Barry N BN Zhao Xilin X Xu Ye Y Li Qingge Q
Journal of clinical microbiology 20160622 9
We report here a ligation-based spoligotyping that can identify unamplified spacers in membrane-based spoligotyping due to asymmetric insertion of IS6110 in the direct repeat locus. Our typing yielded 84.4% (411/487) concordance with traditional typing and 100% (487/487) accuracy when confirmed by DNA sequencing. ...[more]
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