Rapid and specific detection of Lassa virus by reverse transcription-PCR coupled with oligonucleotide array hybridization.
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ABSTRACT:
SUBMITTER: Olschlager S
PROVIDER: S-EPMC3405593 | biostudies-literature | 2012 Jul
REPOSITORIES: biostudies-literature
Publications
Rapid and specific detection of Lassa virus by reverse transcription-PCR coupled with oligonucleotide array hybridization.
Olschläger Stephan S Günther Stephan S
Journal of clinical microbiology 20120425 7
To facilitate sequence-specific detection of DNA amplified in a diagnostic reverse transcription (RT)-PCR for Lassa virus, we developed an array featuring 47 oligonucleotide probes for post-PCR hybridization of the amplicons. The array procedure may be performed with low-tech equipment and does not take longer than agarose gel detection. ...[more]
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