Ontology highlight
ABSTRACT:
SUBMITTER: Zhang ZW
PROVIDER: S-EPMC7122606 | biostudies-literature | 2005
REPOSITORIES: biostudies-literature
Zhang Zhi-wei ZW Zhou Yi-ming YM Zhang Yan Y Guo Yong Y Tao Sheng-ce SC Li Ze Z Zhang Qiong Q Cheng Jing J
Methods in molecular medicine 20050101
We have developed a sensitive method for the detection of specific genes simultaneously. First, DNA was amplified by a novel asymmetric multiplex PCR with universal primer(s). Second, the 6-carboxytetramethylrhodamine (TAMRA)-labeled PCR products were hybridized specifically with oligonucleotide microarrays. Finally, matched duplexes were detected by using a laser-induced fluorescence scanner. The usefulness of this method was illustrated by analyzing severe acute respiratory syndrome (SARS) cor ...[more]