Unknown

Dataset Information

0

Presynaptic CB(1) cannabinoid receptors control frontocortical serotonin and glutamate release--species differences.


ABSTRACT: Both the serotonergic and endocannabinoid systems modulate frontocortical glutamate release; thus they are well positioned to participate in the pathogenesis of psychiatric disorders. With the help of fluorescent and confocal microscopy, we localized the CB(1) cannabinoid receptor (CB(1)R) in VGLUT1- and 2- (i.e. glutamatergic) and serotonin transporter- (i.e. serotonergic) -positive fibers and nerve terminals in the mouse and rat frontal cortex. CB(1)R activation by the synthetic agonists, WIN55212-2 (1 ?M) and R-methanandamide (1 ?M) inhibited the simultaneously measured evoked Ca(2+)-dependent release of [(14)C]glutamate and [(3)H]serotonin from frontocortical nerve terminals of Wistar rats, in a fashion sensitive to the CB(1)R antagonists, O-2050 (1 ?M) and LY320135 (5 ?M). CB(1)R agonists also inhibited the evoked release of [(14)C]glutamate in C57BL/6J mice in a reversible fashion upon washout. Interestingly, the evoked release of [(14)C]glutamate and [(3)H]serotonin was significantly greater in the CB(1)R knockout CD-1 mice. Furthermore, CB(1)R binding experiments revealed similar frontocortical CB(1)R density in the rat and the CD-1 mouse. Still, the evoked release of [(3)H]serotonin was modulated by neither CB(1)R agonists nor antagonists in wild-type CD-1 or C57BL/6J mice. Altogether, this is the first study to demonstrate functional presynaptic CB(1)Rs in frontocortical glutamatergic and serotonergic terminals, revealing species differences.

SUBMITTER: Ferreira SG 

PROVIDER: S-EPMC3408788 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature

altmetric image

Publications

Presynaptic CB(1) cannabinoid receptors control frontocortical serotonin and glutamate release--species differences.

Ferreira Samira G SG   Teixeira Filipe M FM   Garção Pedro P   Agostinho Paula P   Ledent Catherine C   Cortes Luísa L   Mackie Ken K   Köfalvi Attila A  

Neurochemistry international 20120517 2


Both the serotonergic and endocannabinoid systems modulate frontocortical glutamate release; thus they are well positioned to participate in the pathogenesis of psychiatric disorders. With the help of fluorescent and confocal microscopy, we localized the CB(1) cannabinoid receptor (CB(1)R) in VGLUT1- and 2- (i.e. glutamatergic) and serotonin transporter- (i.e. serotonergic) -positive fibers and nerve terminals in the mouse and rat frontal cortex. CB(1)R activation by the synthetic agonists, WIN5  ...[more]

Similar Datasets

| S-EPMC7457419 | biostudies-literature
| S-EPMC3790928 | biostudies-literature
| S-EPMC6979073 | biostudies-literature
| S-EPMC8281444 | biostudies-literature
| S-EPMC3164946 | biostudies-literature
| S-EPMC6729523 | biostudies-literature
| S-EPMC2993256 | biostudies-literature
| S-EPMC8122071 | biostudies-literature
| S-EPMC6433645 | biostudies-literature
| S-EPMC3358745 | biostudies-literature