Ontology highlight
ABSTRACT:
SUBMITTER: Thompson DB
PROVIDER: S-EPMC3408882 | biostudies-literature | 2012 Jul
REPOSITORIES: biostudies-literature
Thompson David B DB Villaseñor Roberto R Dorr Brent M BM Zerial Marino M Liu David R DR
Chemistry & biology 20120701 7
Supercharged proteins (SCPs) can deliver functional macromolecules into the cytoplasm of mammalian cells more potently than unstructured cationic peptides. Thus far, neither the structural features of SCPs that determine their delivery effectiveness nor their intracellular fate postendocytosis, has been studied. Using a large set of supercharged GFP (scGFP) variants, we found that the level of cellular uptake is sigmoidally related to net charge and that scGFPs enter cells through multiple pathw ...[more]