Ontology highlight
ABSTRACT:
SUBMITTER: Pitchiaya S
PROVIDER: S-EPMC3410386 | biostudies-literature | 2012 Aug
REPOSITORIES: biostudies-literature
EMBO reports 20120612 8
MicroRNAs (miRNAs) associate with components of the RNA-induced silencing complex (RISC) to assemble on mRNA targets and regulate protein expression in higher eukaryotes. Here we describe a method for the intracellular single-molecule, high-resolution localization and counting (iSHiRLoC) of miRNAs. Microinjected, singly fluorophore-labelled, functional miRNAs were tracked within diffusing particles, a majority of which contained single such miRNA molecules. Mobility and mRNA-dependent assembly c ...[more]