Ontology highlight
ABSTRACT:
SUBMITTER: Aboye TL
PROVIDER: S-EPMC3411040 | biostudies-literature | 2012 Aug
REPOSITORIES: biostudies-literature
The Journal of biological chemistry 20120614 32
Here, we review the use of different biochemical approaches for biological synthesis of circular or backbone-cyclized proteins and peptides. These methods allow the production of circular polypeptides either in vitro or in vivo using standard recombinant DNA expression techniques. Protein circularization can significantly impact protein engineering and research in protein folding. Basic polymer theory predicts that circularization should lead to a net thermodynamic stabilization of a folded prot ...[more]