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Investigating Mithramycin deoxysugar biosynthesis: enzymatic total synthesis of TDP-D-olivose.


ABSTRACT: Mix'n'match: Enzymatic total synthesis of TDP-D-olivose was achieved, starting from TDP-4-keto-6-deoxy-D-glucose, by combining three pathway enzymes with one cofactor-regenerating enzyme. The results also revealed that MtmC is a bifunctional enzyme that can perform a 4-ketoreduction necessary for D-olivose biosynthesis besides the previously found C-methyltransfer for D-mycarose biosynthesis.

SUBMITTER: Wang G 

PROVIDER: S-EPMC3412565 | biostudies-literature |

REPOSITORIES: biostudies-literature

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