Ontology highlight
ABSTRACT:
SUBMITTER: Yaakov G
PROVIDER: S-EPMC3413326 | biostudies-literature | 2012 Jul
REPOSITORIES: biostudies-literature
Yaakov Gilad G Thorn Kurt K Thorn Kurt K Morgan David O DO
Developmental cell 20120701 1
In anaphase, sister chromatids separate abruptly and are then segregated by the mitotic spindle. The protease separase triggers sister separation by cleaving the Scc1/Mcd1 subunit of the cohesin ring that holds sisters together. Polo-kinase phosphorylation of Scc1 promotes its cleavage, but the underlying regulatory circuits are unclear. We developed a separase biosensor in Saccharomyces cerevisiae that provides a quantitative indicator of cohesin cleavage in single cells. Separase is abruptly a ...[more]