Ontology highlight
ABSTRACT:
SUBMITTER: Rosen LE
PROVIDER: S-EPMC6858450 | biostudies-literature | 2019 Nov
REPOSITORIES: biostudies-literature
Rosen Laura E LE Klebba Joseph E JE Asfaha Jonathan B JB Ghent Chloe M CM Campbell Melody G MG Cheng Yifan Y Morgan David O DO
Nature communications 20191115 1
Chromosome segregation begins when the cysteine protease, separase, cleaves the Scc1 subunit of cohesin at the metaphase-to-anaphase transition. Separase is inhibited prior to metaphase by the tightly bound securin protein, which contains a pseudosubstrate motif that blocks the separase active site. To investigate separase substrate specificity and regulation, here we develop a system for producing recombinant, securin-free human separase. Using this enzyme, we identify an LPE motif on the Scc1 ...[more]