FRAP analysis: accounting for bleaching during image capture.
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ABSTRACT:
SUBMITTER: Wu J
PROVIDER: S-EPMC3415426 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
Publications
FRAP analysis: accounting for bleaching during image capture.
Wu Jun J Shekhar Nandini N Lele Pushkar P PP Lele Tanmay P TP
PloS one 20120809 8
The analysis of Fluorescence Recovery After Photobleaching (FRAP) experiments involves mathematical modeling of the fluorescence recovery process. An important feature of FRAP experiments that tends to be ignored in the modeling is that there can be a significant loss of fluorescence due to bleaching during image capture. In this paper, we explicitly include the effects of bleaching during image capture in the model for the recovery process, instead of correcting for the effects of bleaching usi ...[more]
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