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SrfJ, a Salmonella type III secretion system effector regulated by PhoP, RcsB, and IolR.

ABSTRACT: Virulence-related type III secretion systems are present in many Gram-negative bacterial pathogens. These complex devices translocate proteins, called effectors, from the bacterium into the eukaryotic host cell. Here, we identify the product of srfJ, a Salmonella enterica serovar Typhimurium gene regulated by SsrB, as a new substrate of the type III secretion system encoded by Salmonella pathogenicity island 2. The N-terminal 20-amino-acid segment of SrfJ was recognized as a functional secretion and translocation signal specific for this system. Transcription of srfJ was positively regulated by the PhoP/PhoQ system in an SsrB-dependent manner and was negatively regulated by the Rcs system in an SsrB-independent manner. A screen for regulators of an srfJ-lacZ transcriptional fusion using the T-POP transposon identified IolR, the regulator of genes involved in myo-inositol utilization, as an srfJ repressor. Our results suggest that SrfJ is synthesized both inside the host, in response to intracellular conditions, and outside the host, in myo-inositol-rich environments.

SUBMITTER: Cordero-Alba M

PROVIDER: S-EPMC3416237 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature

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SrfJ, a Salmonella type III secretion system effector regulated by PhoP, RcsB, and IolR.

Cordero-Alba Mar M Bernal-Bayard Joaquín J Ramos-Morales Francisco F

Journal of bacteriology 20120601 16

Virulence-related type III secretion systems are present in many Gram-negative bacterial pathogens. These complex devices translocate proteins, called effectors, from the bacterium into the eukaryotic host cell. Here, we identify the product of srfJ, a Salmonella enterica serovar Typhimurium gene regulated by SsrB, as a new substrate of the type III secretion system encoded by Salmonella pathogenicity island 2. The N-terminal 20-amino-acid segment of SrfJ was recognized as a functional secretion ...[more]

PMID: 22661691

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Dataset Information

SrfJ, a Salmonella type III secretion system effector regulated by PhoP, RcsB, and IolR.

Publications

SrfJ, a Salmonella type III secretion system effector regulated by PhoP, RcsB, and IolR.

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