Synthesis of chiral cyanohydrins by recombinant Escherichia coli cells in a micro-aqueous reaction system.
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ABSTRACT:
SUBMITTER: Scholz KE
PROVIDER: S-EPMC3416370 | biostudies-literature | 2012 Jul
REPOSITORIES: biostudies-literature
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Synthesis of chiral cyanohydrins by recombinant Escherichia coli cells in a micro-aqueous reaction system.
Scholz Kathrin Emmi KE Okrob Daniel D Kopka Benita B Grünberger Alexander A Pohl Martina M Jaeger Karl-Erich KE Krauss Ulrich U
Applied and environmental microbiology 20120427 14
Synthesis of chiral cyanohydrins is performed in a monophasic micro-aqueous reaction system using whole recombinant Escherichia coli cells expressing the Arabidopsis thaliana hydroxynitrile lyase (AtHNL). Microscopy studies employing a fusion of AtHNL with a flavin-based fluorescent protein (FbFP) reveal that the cells remain intact in the reaction system. ...[more]
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