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Development of an efficient in vivo system (Pjunc-TpaseIS1223) for random transposon mutagenesis of Lactobacillus casei.

ABSTRACT: The random transposon mutagenesis system P(junc)-TpaseIS(1223) is composed of plasmids pVI129, expressing IS1223 transposase, and pVI110, a suicide transposon plasmid carrying the P(junc) sequence, the substrate of the IS1223 transposase. This system is particularly efficient in Lactobacillus casei, as more than 10,000 stable, random mutants were routinely obtained via electroporation.

SUBMITTER: Licandro-Seraut H

PROVIDER: S-EPMC3416393 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature

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Development of an efficient in vivo system (Pjunc-TpaseIS1223) for random transposon mutagenesis of Lactobacillus casei.

Licandro-Seraut Hélène H Brinster Sophie S van de Guchte Maarten M Scornec Hélène H Maguin Emmanuelle E Sansonetti Philippe P Cavin Jean-François JF Serror Pascale P

Applied and environmental microbiology 20120518 15

The random transposon mutagenesis system P(junc)-TpaseIS(1223) is composed of plasmids pVI129, expressing IS1223 transposase, and pVI110, a suicide transposon plasmid carrying the P(junc) sequence, the substrate of the IS1223 transposase. This system is particularly efficient in Lactobacillus casei, as more than 10,000 stable, random mutants were routinely obtained via electroporation. ...[more]

PMID: 22610425

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