Ontology highlight
ABSTRACT:
SUBMITTER: Samhita L
PROVIDER: S-EPMC3420168 | biostudies-literature | 2012 Aug
REPOSITORIES: biostudies-literature
Samhita Laasya L Shetty Sunil S Varshney Umesh U
Proceedings of the National Academy of Sciences of the United States of America 20120724 32
Of all tRNAs, initiator tRNA is unique in its ability to start protein synthesis by directly binding the ribosomal P-site. This ability is believed to derive from the almost universal presence of three consecutive G-C base (3G-C) pairs in the anticodon stem of initiator tRNA. Consistent with the hypothesis, a plasmid-borne initiator tRNA with one, two, or all 3G-C pairs mutated displays negligible initiation activity when tested in a WT Escherichia coli cell. Given this, the occurrence of unconv ...[more]