Ontology highlight
ABSTRACT:
SUBMITTER: Davis MP
PROVIDER: S-EPMC3422281 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
Davis Matthew P A MP Abreu-Goodger Cei C van Dongen Stijn S Lu Dong D Tate Peri H PH Bartonicek Nenad N Kutter Claudia C Liu Pentao P Skarnes William C WC Enright Anton J AJ Dunham Ian I
PloS one 20120817 8
Small RNAs such as microRNAs play important roles in embryonic stem cell maintenance and differentiation. A broad range of microRNAs is expressed in embryonic stem cells while only a fraction of their targets have been identified. We have performed large-scale identification of embryonic stem cell microRNA targets using a murine embryonic stem cell line deficient in the expression of Dgcr8. These cells are heavily depleted for microRNAs, allowing us to reintroduce specific microRNA duplexes and ...[more]