Ontology highlight
ABSTRACT:
SUBMITTER: Kim J
PROVIDER: S-EPMC3424517 | biostudies-literature | 2011 Dec
REPOSITORIES: biostudies-literature
Nature methods 20111204 1
The GFP reconstitution across synaptic partners (GRASP) technique, based on functional complementation between two nonfluorescent GFP fragments, can be used to detect the location of synapses quickly, accurately and with high spatial resolution. The method has been previously applied in the nematode and the fruit fly but requires substantial modification for use in the mammalian brain. We developed mammalian GRASP (mGRASP) by optimizing transmembrane split-GFP carriers for mammalian synapses. Us ...[more]