Ontology highlight
ABSTRACT:
SUBMITTER: Izquierdo-Serra M
PROVIDER: S-EPMC6170329 | biostudies-literature | 2018 Oct
REPOSITORIES: biostudies-literature
Izquierdo-Serra Mercè M Hirtz Jan J JJ Shababo Ben B Yuste Rafael R
iScience 20180913
The development of optical methods to activate neurons with single-cell resolution has enabled systematic mapping of inhibitory connections. In contrast, optical mapping of excitatory connections between pyramidal neurons (PCs) has been a major challenge due to their high densities in cortical tissue and their weak and stochastic connectivity. Here we present an optogenetic two-photon mapping method in mouse neocortical slices by activating PCs with the red-shifted opsin C1V1 while recording pos ...[more]