Ontology highlight
ABSTRACT:
SUBMITTER: Mazza D
PROVIDER: S-EPMC3424588 | biostudies-literature | 2012 Aug
REPOSITORIES: biostudies-literature
Mazza Davide D Abernathy Alice A Golob Nicole N Morisaki Tatsuya T McNally James G JG
Nucleic acids research 20120725 15
Live-cell measurement of protein binding to chromatin allows probing cellular biochemistry in physiological conditions, which are difficult to mimic in vitro. However, different studies have yielded widely discrepant predictions, and so it remains uncertain how to make the measurements accurately. To establish a benchmark we measured binding of the transcription factor p53 to chromatin by three approaches: fluorescence recovery after photobleaching (FRAP), fluorescence correlation spectroscopy ( ...[more]