Ontology highlight
ABSTRACT:
SUBMITTER: Grotenbreg GM
PROVIDER: S-EPMC3427782 | biostudies-literature | 2007 Jul
REPOSITORIES: biostudies-literature
Grotenbreg Gijsbert M GM Nicholson Melissa J MJ Fowler Kevin D KD Wilbuer Kathrin K Octavio Leah L Yang Maxine M Chakraborty Arup K AK Ploegh Hidde L HL Wucherpfennig Kai W KW
The Journal of biological chemistry 20070524 29
DM catalyzes the exchange of peptides bound to Class II major histocompatibility complex (MHC) molecules. Because the dissociation and association components of the overall reaction are difficult to separate, a detailed mechanism of DM catalysis has long resisted elucidation. UV irradiation of DR molecules loaded with a photocleavable peptide (caged Class II MHC molecules) enabled synchronous and verifiable evacuation of the peptide-binding groove and tracking of early binding events in real tim ...[more]