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Automated whole-cell patch-clamp electrophysiology of neurons in vivo.

ABSTRACT: Whole-cell patch-clamp electrophysiology of neurons is a gold-standard technique for high-fidelity analysis of the biophysical mechanisms of neural computation and pathology, but it requires great skill to perform. We have developed a robot that automatically performs patch clamping in vivo, algorithmically detecting cells by analyzing the temporal sequence of electrode impedance changes. We demonstrate good yield, throughput and quality of automated intracellular recording in mouse cortex and hippocampus.

SUBMITTER: Kodandaramaiah SB

PROVIDER: S-EPMC3427788 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature

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Automated whole-cell patch-clamp electrophysiology of neurons in vivo.

Kodandaramaiah Suhasa B SB Franzesi Giovanni Talei GT Chow Brian Y BY Boyden Edward S ES Forest Craig R CR

Nature methods 20120506 6

Whole-cell patch-clamp electrophysiology of neurons is a gold-standard technique for high-fidelity analysis of the biophysical mechanisms of neural computation and pathology, but it requires great skill to perform. We have developed a robot that automatically performs patch clamping in vivo, algorithmically detecting cells by analyzing the temporal sequence of electrode impedance changes. We demonstrate good yield, throughput and quality of automated intracellular recording in mouse cortex and h ...[more]

PMID: 22561988

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