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Multiplex PCR for rapid detection of genes encoding class A carbapenemases.

ABSTRACT: In recent years, there have been increasing reports of KPC-producing Klebsiella pneumoniae in Korea. The modified Hodge test can be used as a phenotypic screening test for class A carbapenamase (CAC)-producing clinical isolates; however, it does not distinguish between carbapenemase types. The confirmation of type of CAC is important to ensure optimal therapy and to prevent transmission. This study applied a novel multiplex PCR assay to detect and differentiate CAC genes in a single reaction. Four primer pairs were designed to amplify fragments encoding 4 CAC families (SME, IMI/NMC-A, KPC, and GES). The multiplex PCR detected all genes tested for 4 CAC families that could be differentiated by fragment size according to gene type. This multiplex PCR offers a simple and useful approach for detecting and distinguishing CAC genes in carbapenem-resistant strains that are metallo-?-lactamase nonproducers.

SUBMITTER: Hong SS

PROVIDER: S-EPMC3427824 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature

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Multiplex PCR for rapid detection of genes encoding class A carbapenemases.

Hong Sang Sook SS Kim Kyeongmi K Huh Ji Young JY Jung Bochan B Kang Myung Seo MS Hong Seong Geun SG

Annals of laboratory medicine 20120813 5

In recent years, there have been increasing reports of KPC-producing Klebsiella pneumoniae in Korea. The modified Hodge test can be used as a phenotypic screening test for class A carbapenamase (CAC)-producing clinical isolates; however, it does not distinguish between carbapenemase types. The confirmation of type of CAC is important to ensure optimal therapy and to prevent transmission. This study applied a novel multiplex PCR assay to detect and differentiate CAC genes in a single reaction. Fo ...[more]

PMID: 22950072

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