Ontology highlight
ABSTRACT:
SUBMITTER: Marty MT
PROVIDER: S-EPMC3428216 | biostudies-literature | 2012 Jul
REPOSITORIES: biostudies-literature
Marty Michael T MT Sloan Courtney D Kuhnline CD Bailey Ryan C RC Sligar Stephen G SG
Analytical chemistry 20120622 13
Conventional methods to probe the binding kinetics of macromolecules at biosensor surfaces employ a stepwise titration of analyte concentrations and measure the association and dissociation to the immobilized ligand at each concentration level. It has previously been shown that kinetic rates can be measured in a single step by monitoring binding as the analyte concentration increases over time in a linear gradient. We report here the application of nonlinear analyte concentration gradients for d ...[more]