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Bifunctional Janus microparticles with spatially segregated proteins.

ABSTRACT: We present a fabrication process to create bifunctional microparticles displaying two distinct proteins that are spatially segregated onto the surface hemispheres. Silica and polystyrene microparticles with 2.0, 4.1, and 4.7 ?m diameters are processed with metal deposition to form two chemically distinct and segregated hemispheres. The surface of each hemisphere is then separately derivatized with biological proteins using different chemical conjugation strategies. These bifunctional Janus particles possess biologically relevant, native conformation proteins attached to a biologically unreactive and safe substrate. They also display high densities of each type of protein which may enable a range of capabilities that monofunctional particles cannot, such as improved targeting of drugs and bioimaging agents.

SUBMITTER: Tang JL

PROVIDER: S-EPMC3428262 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature

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Bifunctional Janus microparticles with spatially segregated proteins.

Tang Jennifer L JL Schoenwald Kipp K Potter Daniel D White David D Sulchek Todd T

Langmuir : the ACS journal of surfaces and colloids 20120618 26

We present a fabrication process to create bifunctional microparticles displaying two distinct proteins that are spatially segregated onto the surface hemispheres. Silica and polystyrene microparticles with 2.0, 4.1, and 4.7 μm diameters are processed with metal deposition to form two chemically distinct and segregated hemispheres. The surface of each hemisphere is then separately derivatized with biological proteins using different chemical conjugation strategies. These bifunctional Janus parti ...[more]

PMID: 22624704

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