Ontology highlight
ABSTRACT:
SUBMITTER: Devaraj NK
PROVIDER: S-EPMC3431914 | biostudies-literature | 2005 Jun
REPOSITORIES: biostudies-literature
Devaraj Neal K NK Miller Gregory P GP Ebina Wataru W Kakaradov Boyko B Collman James P JP Kool Eric T ET Chidsey Christopher E D CE
Journal of the American Chemical Society 20050601 24
A chemoselective route to routinely and rapidly attach oligonucleotide probes to well-defined surfaces is presented. Cu(I) tris(benzyltriazolylmethyl)amine-catalyzed coupling of terminal acetylenes to azides on a self-assembled monolayer is used instead of traditional nucleophilic-electrophilic coupling reactions. The reaction proceeds well even in the presence of purposely introduced nucleophilic and electrophilic impurities. The density of oligonucleotide probes can be controlled by controllin ...[more]