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Purification, crystallization and preliminary X-ray analysis of uracil-DNA glycosylase from Sulfolobus tokodaii strain 7.

ABSTRACT: Uracil-DNA glycosylase (UDG) specifically removes uracil from DNA by catalyzing hydrolysis of the N-glycosidic bond, thereby initiating the base-excision repair pathway. Although a number of UDG structures have been determined, the structure of archaeal UDG remains unknown. In this study, a deletion mutant of UDG isolated from Sulfolobus tokodaii strain 7 (stoUDG?) and stoUDG? complexed with uracil were crystallized and analyzed by X-ray crystallography. The crystals were found to belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 52.2, b = 52.3, c = 74.7?Å and a = 52.1, b = 52.2, c = 74.1 Å for apo stoUDG? and stoUDG? complexed with uracil, respectively.

SUBMITTER: Kawai A

PROVIDER: S-EPMC3433208 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature

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Purification, crystallization and preliminary X-ray analysis of uracil-DNA glycosylase from Sulfolobus tokodaii strain 7.

Kawai Akito A Higuchi Shigesada S Tsunoda Masaru M Nakamura Kazuo T KT Miyamoto Shuichi S

Acta crystallographica. Section F, Structural biology and crystallization communications 20120831 Pt 9

Uracil-DNA glycosylase (UDG) specifically removes uracil from DNA by catalyzing hydrolysis of the N-glycosidic bond, thereby initiating the base-excision repair pathway. Although a number of UDG structures have been determined, the structure of archaeal UDG remains unknown. In this study, a deletion mutant of UDG isolated from Sulfolobus tokodaii strain 7 (stoUDGΔ) and stoUDGΔ complexed with uracil were crystallized and analyzed by X-ray crystallography. The crystals were found to belong to the ...[more]

PMID: 22949205

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