Unknown

Dataset Information

0

Global analysis of short RNAs reveals widespread promoter-proximal stalling and arrest of Pol II in Drosophila.


ABSTRACT: Emerging evidence indicates that gene expression in higher organisms is regulated by RNA polymerase II stalling during early transcription elongation. To probe the mechanisms responsible for this regulation, we developed methods to isolate and characterize short RNAs derived from stalled RNA polymerase II in Drosophila cells. Significant levels of these short RNAs were generated from more than one-third of all genes, indicating that promoter-proximal stalling is a general feature of early polymerase elongation. Nucleotide composition of the initially transcribed sequence played an important role in promoting transcriptional stalling by rendering polymerase elongation complexes highly susceptible to backtracking and arrest. These results indicate that the intrinsic efficiency of early elongation can greatly affect gene expression.

SUBMITTER: Nechaev S 

PROVIDER: S-EPMC3435875 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature

altmetric image

Publications

Global analysis of short RNAs reveals widespread promoter-proximal stalling and arrest of Pol II in Drosophila.

Nechaev Sergei S   Fargo David C DC   dos Santos Gilberto G   Liu Liwen L   Gao Yuan Y   Adelman Karen K  

Science (New York, N.Y.) 20091210 5963


Emerging evidence indicates that gene expression in higher organisms is regulated by RNA polymerase II stalling during early transcription elongation. To probe the mechanisms responsible for this regulation, we developed methods to isolate and characterize short RNAs derived from stalled RNA polymerase II in Drosophila cells. Significant levels of these short RNAs were generated from more than one-third of all genes, indicating that promoter-proximal stalling is a general feature of early polyme  ...[more]

Similar Datasets

2009-12-11 | E-GEOD-18643 | biostudies-arrayexpress
2009-12-11 | GSE18643 | GEO
| S-EPMC2396556 | biostudies-literature
| S-EPMC2492738 | biostudies-literature
2019-06-18 | GSE129235 | GEO
2019-06-18 | GSE113470 | GEO
| S-EPMC5737879 | biostudies-literature
| S-EPMC7402197 | biostudies-literature
| S-EPMC7229826 | biostudies-literature
| S-EPMC7244574 | biostudies-literature