Ontology highlight
ABSTRACT:
SUBMITTER: Nilson KA
PROVIDER: S-EPMC5737879 | biostudies-literature | 2017 Nov
REPOSITORIES: biostudies-literature
Nilson Kyle A KA Lawson Christine K CK Mullen Nicholas J NJ Ball Christopher B CB Spector Benjamin M BM Meier Jeffery L JL Price David H DH
Nucleic acids research 20171101 19
Oxidative stress has pervasive effects on cells but how they respond transcriptionally upon the initial insult is incompletely understood. We developed a nuclear walk-on assay that semi-globally quantifies nascent transcripts in promoter-proximal paused RNA polymerase II (Pol II). Using this assay in conjunction with ChIP-Seq, in vitro transcription, and a chromatin retention assay, we show that within a minute, hydrogen peroxide causes accumulation of Pol II near promoters and enhancers that ca ...[more]