Ontology highlight
ABSTRACT:
SUBMITTER: Chikwana VM
PROVIDER: S-EPMC3436371 | biostudies-literature | 2012 Aug
REPOSITORIES: biostudies-literature
Chikwana Vimbai M VM Stec Boguslaw B Lee Bobby W K BW de Crécy-Lagard Valérie V Iwata-Reuyl Dirk D Swairjo Manal A MA
The Journal of biological chemistry 20120711 36
The enzyme QueF catalyzes the reduction of the nitrile group of 7-cyano-7-deazaguanine (preQ(0)) to 7-aminomethyl-7-deazaguanine (preQ(1)), the only nitrile reduction reaction known in biology. We describe here two crystal structures of Bacillus subtilis QueF, one of the wild-type enzyme in complex with the substrate preQ(0), trapped as a covalent thioimide, a putative intermediate in the reaction, and the second of the C55A mutant in complex with the substrate preQ(0) bound noncovalently. The Q ...[more]