Evaluation of the compact high-field orbitrap for top-down proteomics of human cells.
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ABSTRACT:
SUBMITTER: Ahlf DR
PROVIDER: S-EPMC3437942 | biostudies-literature | 2012 Aug
REPOSITORIES: biostudies-literature
Publications
Evaluation of the compact high-field orbitrap for top-down proteomics of human cells.
Ahlf Dorothy R DR Compton Philip D PD Tran John C JC Early Bryan P BP Thomas Paul M PM Kelleher Neil L NL
Journal of proteome research 20120713 8
Mass spectrometry based proteomics generally seeks to identify and fully characterize protein species with high accuracy and throughput. Recent improvements in protein separation have greatly expanded the capacity of top-down proteomics (TDP) to identify a large number of intact proteins. To date, TDP has been most tightly associated with Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry. Here, we couple the improved separations to a Fourier-transform instrument based not on I ...[more]
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