Ontology highlight
ABSTRACT:
SUBMITTER: Catherman AD
PROVIDER: S-EPMC3565750 | biostudies-literature | 2013 Feb
REPOSITORIES: biostudies-literature
Analytical chemistry 20130123 3
The interrogation of intact integral membrane proteins has long been a challenge for biological mass spectrometry. Here, we demonstrate the application of top down mass spectrometry to whole membrane proteins below 60 kDa with up to 8 transmembrane helices. Analysis of enriched mitochondrial membrane preparations from human cells yielded identification of 83 integral membrane proteins, along with 163 membrane-associated or soluble proteins, with a median q value of 3 × 10(-10). An analysis of ma ...[more]