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Direct visualization of specifically modified extracellular glycans in living animals.

ABSTRACT: Modification patterns of heparan sulfate coordinate protein function in metazoans, yet in vivo imaging of such non-genetically encoded structures has been impossible. Here we report a transgenic method in Caenorhabditis elegans that allows direct live imaging of specific heparan sulfate modification patterns. This experimental approach reveals a dynamic and cell-specific heparan sulfate landscape and could in principle be adapted to visualize and analyze any extracellular molecule in vivo.

SUBMITTER: Attreed M

PROVIDER: S-EPMC3437987 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature

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Direct visualization of specifically modified extracellular glycans in living animals.

Attreed Matthew M Desbois Muriel M van Kuppevelt Toin H TH Bülow Hannes E HE

Nature methods 20120401 5

Modification patterns of heparan sulfate coordinate protein function in metazoans, yet in vivo imaging of such non-genetically encoded structures has been impossible. Here we report a transgenic method in Caenorhabditis elegans that allows direct live imaging of specific heparan sulfate modification patterns. This experimental approach reveals a dynamic and cell-specific heparan sulfate landscape and could in principle be adapted to visualize and analyze any extracellular molecule in vivo. ...[more]

PMID: 22466794

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