Error-correcting barcoded primers for pyrosequencing hundreds of samples in multiplex.
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ABSTRACT:
SUBMITTER: Hamady M
PROVIDER: S-EPMC3439997 | biostudies-literature | 2008 Mar
REPOSITORIES: biostudies-literature
Publications
Error-correcting barcoded primers for pyrosequencing hundreds of samples in multiplex.
Hamady Micah M Walker Jeffrey J JJ Harris J Kirk JK Gold Nicholas J NJ Knight Rob R
Nature methods 20080210 3
We constructed error-correcting DNA barcodes that allow one run of a massively parallel pyrosequencer to process up to 1,544 samples simultaneously. Using these barcodes we processed bacterial 16S rRNA gene sequences representing microbial communities in 286 environmental samples, corrected 92% of sample assignment errors, and thus characterized nearly as many 16S rRNA genes as have been sequenced to date by Sanger sequencing. ...[more]
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