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Variation in genes related to cochlear biology is strongly associated with adult-onset deafness in border collies.

ABSTRACT: Domestic dogs can suffer from hearing losses that can have profound impacts on working ability and quality of life. We have identified a type of adult-onset hearing loss in Border Collies that appears to have a genetic cause, with an earlier age of onset (3-5 years) than typically expected for aging dogs (8-10 years). Studying this complex trait within pure breeds of dog may greatly increase our ability to identify genomic regions associated with risk of hearing impairment in dogs and in humans. We performed a genome-wide association study (GWAS) to detect loci underlying adult-onset deafness in a sample of 20 affected and 28 control Border Collies. We identified a region on canine chromosome 6 that demonstrates extended support for association surrounding SNP Chr6.25819273 (p-value = 1.09 × 10(-13)). To further localize disease-associated variants, targeted next-generation sequencing (NGS) of one affected and two unaffected dogs was performed. Through additional validation based on targeted genotyping of additional cases (n = 23 total) and controls (n = 101 total) and an independent replication cohort of 16 cases and 265 controls, we identified variants in USP31 that were strongly associated with adult-onset deafness in Border Collies, suggesting the involvement of the NF-?B pathway. We found additional support for involvement of RBBP6, which is critical for cochlear development. These findings highlight the utility of GWAS-guided fine-mapping of genetic loci using targeted NGS to study hereditary disorders of the domestic dog that may be analogous to human disorders.

SUBMITTER: Yokoyama JS

PROVIDER: S-EPMC3441646 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature

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Variation in genes related to cochlear biology is strongly associated with adult-onset deafness in border collies.

Yokoyama Jennifer S JS Lam Ernest T ET Ruhe Alison L AL Erdman Carolyn A CA Robertson Kathryn R KR Webb Aubrey A AA Williams D Colette DC Chang Melanie L ML Hytönen Marjo K MK Lohi Hannes H Hamilton Steven P SP Neff Mark W MW

PLoS genetics 20120913 9

Domestic dogs can suffer from hearing losses that can have profound impacts on working ability and quality of life. We have identified a type of adult-onset hearing loss in Border Collies that appears to have a genetic cause, with an earlier age of onset (3-5 years) than typically expected for aging dogs (8-10 years). Studying this complex trait within pure breeds of dog may greatly increase our ability to identify genomic regions associated with risk of hearing impairment in dogs and in humans. ...[more]

PMID: 23028339

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Dataset Information

Variation in genes related to cochlear biology is strongly associated with adult-onset deafness in border collies.

Publications

Variation in genes related to cochlear biology is strongly associated with adult-onset deafness in border collies.

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