A temperature-sensitive mutation in the dnaE gene of Caulobacter crescentus that prevents initiation of DNA replication but not ongoing elongation of DNA.
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ABSTRACT:
SUBMITTER: Lo T
PROVIDER: S-EPMC344199 | biostudies-literature | 2004 Feb
REPOSITORIES: biostudies-literature
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A temperature-sensitive mutation in the dnaE gene of Caulobacter crescentus that prevents initiation of DNA replication but not ongoing elongation of DNA.
Lo Teresa T van Der Schalie Ellena E Werner Theresa T Brun Yves V YV Din Neena N
Journal of bacteriology 20040201 4
A genetic screen for cell division cycle mutants of Caulobacter crescentus identified a temperature-sensitive DNA replication mutant. Genetic complementation experiments revealed a mutation within the dnaE gene, encoding the alpha-catalytic subunit of DNA polymerase III holoenzyme. Sequencing of the temperature-sensitive dnaE allele indicated a single base pair substitution resulting in a change from valine to glutamic acid within the C-terminal portion of the protein. This mutation lies in a re ...[more]
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