Ontology highlight
ABSTRACT:
SUBMITTER: Lapenta F
PROVIDER: S-EPMC4822814 | biostudies-literature | 2016
REPOSITORIES: biostudies-literature
PloS one 20160406 4
DNA Polymerases generate pyrophosphate every time they catalyze a step of DNA elongation. This elongation reaction is generally believed as thermodynamically favoured by the hydrolysis of pyrophosphate, catalyzed by inorganic pyrophosphatases. However, the specific action of inorganic pyrophosphatases coupled to DNA replication in vivo was never demonstrated. Here we show that the Polymerase-Histidinol-Phosphatase (PHP) domain of Escherichia coli DNA Polymerase III α subunit features pyrophospha ...[more]